[R25] How Reversed-Phase Liquid Chromatography Works

M.R. Schure, J.L. Rafferty, L. Zhang, and J.I. Siepmann

LC GC North America 31, 630-637 (2013)

Publication Abstract

The keys to understanding reversed-phase liquid chromatography (LC) are provided at the molecular mechanism level as determined by high accuracy molecular simulation. The essential features of C18 stationaryphase chains in contact with methanol–water and acetonitrile–water mixtures are discussed in the context of bonded-chain geometry, spatial distribution of alkane and alcohol solutes, retention mechanism, and retention thermodynamics. This tutorial is intended to be applicable to a wide audience ranging from occasional users of liquid chromatography to separation scientists.

How Reversed-Phase Liquid Chromatography Works